Monday 24 February 2020

Blastocyst Culture :-

Blastocyst culture refers to growing the embryos in the laboratory for two more days at which point they are referred to as blastocyst embryos. Blastocyst transfer simply means that the blastocyst embryos are transferred to the woman’s uterus on day 5 in exactly the same way that day 3 embryos .

Three important factors previously limited the number of embryos that could survive to this stage (development stage) :-

First, the "health" of any embryo will decided its ability to continue to grow and divide. Several eggs may have initially fertilized, but only a few will progress to the four-cell stage on day two in culture, fewer still to the eight-cell stage on day three in culture, and even fewer will develop into blastocysts. This stage can be called as "survival of the fittest."

The second, limiting factor in preventing continued growth of embryos in culture had been the culture media Or blastocyst media.. These are rich in life-sustaining nutrients that give the embryos every chance to reach there full inherent potential. As a result , embryos will now be exposed to specific media that are designed to meet the nutritional requirements of each stage of development.

Third, even with embryos of good growth potential and optimal culture media, the experience and expertise of the laboratory embryologists doing this important work is critical to achieving good blastocyst development.

Advantages of blastocyst :-

1) It makes a better selection of embryos possible. Embryos which reach this stage offer a better implantation capacity. It is for that reason that we limit transfers to 1 blastocyst (or maximum 2), reducing the risk of multiple births.

2) It allows a more physiological synchronisation between the embryonic phase and the responsiveness of the endometrium.

3) It facilitates the correct supervision of embryo development and the transfer of only the embryos selected by Nature to reach an optimum state of development, which in turn allows for a higher implantation rate.

Disadvantage of blastocyst transfer :-

4.However, if the culture environment is suboptimal, delayed embryo development and even embryonic arrest will occur – at least in some cases. Therefore, if the culture system and quality control in an IVF lab are inconsistent, good results will not be obtained. The only disadvantage is lack of facilities and knowledge to embryologists.


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